The objective of the project was to examine the effects of CBD on canine cancer cell growth proliferation with aims towards understanding the signaling pathways involved and the apoptotic response related to CBD. A second aim was to understand the influence of CBD co-treatment with chemotherapeutics such as doxorubicin and vincristine. Both of these aims are outlined and presented in the publication listed above and is a comprehensive review of our results from this project. Investigators found that CBD is effective at hindering cell proliferation and induction of autophagy and apoptosis rapidly across neoplastic cell lines. Further clinical trials are needed to understand its efficacy and interactions with traditional chemotherapy.
Over the last 6 months we have completed some of the mitochondrial data that was no part of the initial publication and was the third aim of our study to further examine mitochondrial influence on the overall apoptotic response. Flow cytometry assays are not showing that mitochondrial perturbations do not supersede the apoptotic response in these cell lines and that CBD treatment does not act as a potent free radical scavenger or generator that may be influencing mitochondrial function during CBD treatment.
Final Update – Examination of the Effects of Cannabidiol on Canine Neoplastic Cell
Apoptosis/Autophagy and Potential for Chemotherapy Resistance or Sensitivity