Research Update 02945-MOU MY3: Understanding the genetic basis of Addison’s disease in Portuguese Water Dogs

Over the first 2.5 years of this study, we have focused primarily on sample collection and running an initial set of genetics experiments. We collected samples mostly through PWD-related channels working directly with our collaborators in the PWDF/PWDCA. We also attended the PWD National Specialty in September 2021 and August 2022 to promote the study and collect samples, and we established a collaboration with Dr. Anita Oberbauer at UC Davis to share samples that she had already collected. To date, we have in hand 134 samples from affected PWDs and 169 samples from unaffected PWDs. Continue reading “Research Update 02945-MOU MY3: Understanding the genetic basis of Addison’s disease in Portuguese Water Dogs”

Research Update CHF 02661 MY5: Investigation into Diet-Associated Dilated Cardiomyopathy in Dogs

The study titled “Investigation into Subclinical Diet-Associated Dilated Cardiomyopathy in Four Dog Breeds” has resulted in 4 peer-reviewed manuscripts published in the Journal of Veterinary Internal Medicine, the American Journal of Veterinary Research and Scientific Reports.

The results of this work demonstrated higher levels of cardiac troponin I in dogs eating grain-free (GF) dog foods or foods that have peas, lentils or potatoes in the top 10 ingredients compared to dogs eating grain-inclusive (GI) dog foods or foods without peas, lentils, or potatoes in the top 10 ingredients. Cardiac troponin I is a blood marker that indicates injury to the heart muscle and therefore this study provides information on how heart disease occurs in some dogs eating GF diets. Additionally, we showed that cardiac troponin I decreased, and an echocardiographic (ultrasound of the heart) measure of contractility improved in dogs fed GF diets that were changed to a GI diet without peas or lentils 1 year after this diet change. This is important information because it shows that apparently healthy dogs can have subtle heart muscle injury that can improve with diet change. Therefore, this data is supportive of causation. Continue reading “Research Update CHF 02661 MY5: Investigation into Diet-Associated Dilated Cardiomyopathy in Dogs”

Research Update CHF 03045-A EY2: Artificial Intelligence and Machine Learning for Diagnosis of Dog Sperm Morphology

Collection of all semen samples required for the study has been completed. Samples from 62 dogs presented to the veterinary hospital at The Ohio State University have been obtained and preserved. At the University of Pennsylvania, slides were prepared, and images acquired using phase-contrast under 1000X magnification. A bank with over 11K images has been created. Continue reading “Research Update CHF 03045-A EY2: Artificial Intelligence and Machine Learning for Diagnosis of Dog Sperm Morphology”

Research Update CHF 03055 EY1: Evaluating Reproductive Diseases in vitro with a 3D Canine Endometrial Organoid Model

Three-dimensional (3D) organoid cell cultures present new opportunities to improve understanding of common reproductive pathologies in the bitch in a laboratory setting rather than using live animals for research. This is an improvement on using research dogs by:
i) addressing important welfare and ethical concerns,
ii) allowing more controlled study of cellular responses under hormonal influence and infectious agents, and
iii) permitting high-throughput evaluation of treatments performed in tandem. Continue reading “Research Update CHF 03055 EY1: Evaluating Reproductive Diseases in vitro with a 3D Canine Endometrial Organoid Model”

Research Update CHF 03053-A MY1: Genome-wide Association Study to Investigate Genetic Markers for Antimicrobial Resistance in Mycoplasma cynos Associated with Canine Infectious Respiratory Disease

We would like to thank AKC CHF for supporting our research on Mycoplasma cynos, which is an emerging respiratory bacterium in dogs. M. cynos is associated with kennel cough and may lead to fatal pneumonia. This bacterium is very complex, and lack of standardized diagnostic and knowledge on antibiotic therapy makes clinical management difficult. Since no vaccine is available, current treatment of M. cynos-associated outbreaks relies on antibiotics. However, the in vitro activity of antibiotics against M. cynos has never been studied and the effectiveness of antibiotic therapy for M. cynos disease is completely unknown. This may lead to unsuccessful treatment and antibiotic
resistance. Continue reading “Research Update CHF 03053-A MY1: Genome-wide Association Study to Investigate Genetic Markers for Antimicrobial Resistance in Mycoplasma cynos Associated with Canine Infectious Respiratory Disease”

Research Update CHF 03055 MY1: Evaluating Reproductive Diseases in vitro with a 3D Canine Endometrial Organoid Model

Three-dimensional (3D) organoid cell cultures present new opportunities to improve understanding of common reproductive pathologies in the bitch in a laboratory setting rather than using live animals for research. This is an improvement on using research dogs by: i) addressing important welfare and ethical concerns, ii) allowing more controlled study of cellular responses due to different hormones and infectious agents, and iii) permitting high-throughput evaluation of treatments performed in tandem. This 3D reproductive organoid cell culture technology has not been attempted in canines prior to these studies. Continue reading “Research Update CHF 03055 MY1: Evaluating Reproductive Diseases in vitro with a 3D Canine Endometrial Organoid Model”

Research Update CHF 03045-A MY2: Artificial Intelligence and Machine Learning for Diagnosis of Dog Sperm Morphology

Collection of all semen samples required for the study has been completed. Samples from 62 dogs presented to the veterinary hospital at The Ohio State University have been obtained and preserved. At the University of Pennsylvania, slides were prepared, and images acquired using phase-contrast under 1000X magnification. A bank with 11,464 images has been created. Continue reading “Research Update CHF 03045-A MY2: Artificial Intelligence and Machine Learning for Diagnosis of Dog Sperm Morphology”

Research Update CHF 02534 EY 4 Update: Clinical Trial for Evaluation of Propranolol and Doxorubicin in the Treatment of Canine Hemangiosarcoma

We have screened 58 dogs for the study. None of the dogs were enrolled due to the detection of metastatic disease during the screening process. Two other dogs were not screened due to the prior use of Yunnan Baiyao beyond the 24-hour limit allowed, and another owner cancelled the morning of the screening for unknown reasons. We have enrolled 19 dogs to date, bringing enrollment to 95% of the dogs proposed for the study. Continue reading “Research Update CHF 02534 EY 4 Update: Clinical Trial for Evaluation of Propranolol and Doxorubicin in the Treatment of Canine Hemangiosarcoma”

Research Update CHF 02806-MOU EY3: Strategic Prevention of Canine Hemangiosarcoma: Lifetime Follow-Up

The goal for this project is to develop a reliable, accessible, and actionable test to identify dogs at risk for hemangiosarcoma during the earliest stages of disease and to use a strategic, rationally designed approach to prevent its occurrence in these high-risk dogs before it becomes clinically detrimental and life-threatening. Continue reading “Research Update CHF 02806-MOU EY3: Strategic Prevention of Canine Hemangiosarcoma: Lifetime Follow-Up”

Research Update CHF 02819 MY3: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses

As previously reported, due to COVID-19 pandemic and backorders of supplies, we had a short delay in starting the study. We have now received all the lab supplies. Screening of Bh SA2 expression genomic library by colony immunoblotting (Aim1) require additional time and research effort to identify potential diagnostic antigens for Canine Bartonelloses. Once we identify potential diagnostic markers, we will evaluate utility of these proteins and develop a reliable ELISA for serodiagnosis of Bartonella infection in dogs. Continue reading “Research Update CHF 02819 MY3: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses”