Research Update CHF 02624 EY3: Embracing Polygenicity of Common Complex Disease in Dogs: Genome-wide Association of Cruciate Ligament Rupture

We have now completed recruitment and marker genotyping of Labrador Retriever dogs under this award. A final single nucleotide polymorphism (SNP) marker data set of 1,006 dogs has been assembled and filtered for data analysis, slightly exceeding what was promised under the award. Continue reading “Research Update CHF 02624 EY3: Embracing Polygenicity of Common Complex Disease in Dogs: Genome-wide Association of Cruciate Ligament Rupture”

Research Update CHF 02759 Mid-year 2: Reprogramming the Tumor Immune Niche in Canine Hemangiosarcoma

We have made significant progress toward Objective 1 and 2 for this project. Over the past six months, we have continued work to examine molecular and functional changes induced by PIK3CA gene mutations in canine HSA cells. Our data reveal that PI3K inhibitors increase DNA damage greater in HSA cells with PIK3CA mutations than cells without the mutations (i.e., wild-type cells), which depends on a drug dose. Continue reading “Research Update CHF 02759 Mid-year 2: Reprogramming the Tumor Immune Niche in Canine Hemangiosarcoma”

Research Update CHF 02428 EY-4: Identifying the Disease-Defining Autoantibodies in Canine Addison’s Disease

The goal of this project is to identify autoantibodies that are present in the blood of dogs who are newly diagnosed with Addison’s disease in three breeds: Standard Poodles, Portuguese Water Dogs, and English Cocker Spaniels. To accomplish these goals, we have been focusing on (1) collecting blood samples from dogs across all three target breeds, and (2) employing methods that allow us to detect these autoantibodies. Continue reading “Research Update CHF 02428 EY-4: Identifying the Disease-Defining Autoantibodies in Canine Addison’s Disease”

Research Update CHF 02783 EY2: Transcriptional Profiling of Canine Soft Tissue Sarcoma

Soft tissue sarcoma (STS) encompasses a number of neoplasms that are derived from mesenchymal cells including fibrosarcoma, myxosarcoma, hemangiopericytoma, and undifferentiated sarcoma. In the dog, STSs arise frequently in the dermis/subcutis and represent up to 15% of the neoplasms in this location. Our primary aim of this grant was to collect cases of canine STS for histologic and gene expression analysis. Continue reading “Research Update CHF 02783 EY2: Transcriptional Profiling of Canine Soft Tissue Sarcoma”

Research Update CHF 02534 MY 3 Update: Clinical Trial for Evaluation of Propranolol and Doxorubicin in the Treatment of Canine Hemangiosarcoma

We opened the trial on July 1, 2019. As of June 27, 2021, we have enrolled 18 dogs in the study and no dose limiting toxicities within the initial 21-day assessment period have been observed. Based on these results, we are continuing to enroll dogs at the highest dose of propranolol (1.3 mg/kg) being tested. Continue reading “Research Update CHF 02534 MY 3 Update: Clinical Trial for Evaluation of Propranolol and Doxorubicin in the Treatment of Canine Hemangiosarcoma”

Research Update CHF 02403-MOU EY-4: Microphthalmia and Delayed Growth Syndrome in the Portuguese Water Dog

The objective of this study is to A) clinicopathologically and molecularly characterize microphthalmia with delayed growth in the Portuguese Water Dog (POWD) and B) develop a DNA-based test to assist breeders with their breeding programs and avoid producing affected dogs. Continue reading “Research Update CHF 02403-MOU EY-4: Microphthalmia and Delayed Growth Syndrome in the Portuguese Water Dog”

Research Update CHF 02819 EY1: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses

During the past 4 years, with support from the American Kennel Club Canine Health Foundation, our research group has documented that current serological assays lack sensitivity, specificity, or both for assessing exposure to Bartonella spp. in dogs. Thus, when used diagnostically for an individual dog or epidemiologically for the detection of Bartonella spp. antibodies in dog populations, results would be inaccurate due to false-negative (poor sensitivity) or false positive (poor specificity) IFA testing. Continue reading “Research Update CHF 02819 EY1: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses”

Research Update CHF 02806-MOU MY2: Strategic Prevention of Canine Hemangiosarcoma: Lifetime Follow-Up

The goal of this project is to develop a reliable, accessible, and actionable test to identify dogs at risk for hemangiosarcoma during the earliest stages of the disease and to use a strategic, rationally designed approach to prevent its occurrence in these high-risk dogs before it becomes clinically detrimental and life-threatening. Continue reading “Research Update CHF 02806-MOU MY2: Strategic Prevention of Canine Hemangiosarcoma: Lifetime Follow-Up”

Research Update CHF 02686-A EY2: Pattern of Thyroid Function Tests during Recovery from Acute Nonthyroidal Illness

To date, 52 cases have been screened for possible study enrollment, of which 13 fulfilled enrollment criteria (~50% of total cases needed). Based on the case enrollment thus far, 11/13 dogs had normalization of total T4 by the 2-week recheck. Continue reading “Research Update CHF 02686-A EY2: Pattern of Thyroid Function Tests during Recovery from Acute Nonthyroidal Illness”