We are on track to accomplish all of our aims for this study. We were able to obtain the initial set of samples on April 26, 2018 so we had a short delay in starting this study. We have now completed all Year I study aims, with the exception of immunohistochemistry and FISH localization of Bartonella organisms within various cell types. An unanticipated complication arose that the mouse monoclonal antibody was no longer being made commercially. B. henselae specific FISH probes have been designed and validation of FISH probes are in-progress. IHC is also in- progress. All qPCR and ddPCR have been completed at this time and samples are waiting for FISH and IHC analysis.
Research Update Mid-year 2 CHF 02553: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens
We proposed to develop a comprehensive method for detection of infectious diseases of dogs, taking the guesswork out of determining which tests to use for diagnosis, and potentially improving disease surveillance because of the comprehensive nature of the test.
Research Update End-yr 2 CHF 02528: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases
Diagnostic tests based on the detection of DNA from harmful organisms in clinical samples have revolutionized veterinary medicine in the last decades. Currently, diagnostic panels for several vectorborne diseases (VBDs) are available through universities and private labs in the USA and abroad. However, the vast majority of results from sick dogs are negative, which frustrates veterinarians and dog owners trying to reach a definitive diagnosis.
Research Update End-yr 1 CHF 02553: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens
We proposed to develop a comprehensive method for detection of infectious diseases of dogs, taking the guesswork out of determining which tests to use for diagnosis, and potentially improving disease surveillance because of the comprehensive nature of the test.
CHF Grant 02383 Final Update: Identifying Cellular Mechanisms of Inflammation During Canine Tick-Borne Diseases
Update on improving tick-borne disease therapy from Dr Petersen.
Research Update Mid-year 1 CHF-02553: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens
Research update for improving diagnostics for Tick-Borne Disease with genetic sequencing.
Research Update End-Year-1 CHF-02528: Developing a Next Generation Diagnostic Platform for TBD
Research update for improving diagnostics for Tick-Borne Disease.
Research Update Mid-Year-2 for CHF-02519: Prevelance of Bartonella Infection in Dogs with Cardiac and Splenic Hemangiosarcomas
Research update from Dr. Breitschwerdt looking at the potential role of the Tick-Borne bacterial infection, bartonella in the initiation and/or progression of hemangiosarcoma.
Research Update End-Year 2 CHF-02383: Identifying Cellular Mechanisms of Inflammation During Canine Tick-Borne Diseases
Research Update from Dr. Petersen into Tick-Borne Disease.
Research Final Update CHF 02287: Enhanced Testing for the Diagnosis of Bartonellosis in Dogs
Research Final Update from Dr. Breitschwerdt to enhance ability to diagnose Bartonellosis.
