Research Update CHF 02819 MY3: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses

As previously reported, due to COVID-19 pandemic and backorders of supplies, we had a short delay in starting the study. We have now received all the lab supplies. Screening of Bh SA2 expression genomic library by colony immunoblotting (Aim1) require additional time and research effort to identify potential diagnostic antigens for Canine Bartonelloses. Once we identify potential diagnostic markers, we will evaluate utility of these proteins and develop a reliable ELISA for serodiagnosis of Bartonella infection in dogs. Continue reading “Research Update CHF 02819 MY3: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses”

Research Update CHF 02819 MY2: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses

We are on track to accomplish all of our aims for this study. Due to COVID-19 pandemic and backorders of supplies, we had a short delay in starting the study. We have now received all the lab supplies. Screening of Bh SA2 expression genomic library by colony immunoblotting (Aim1) require additional time and research effort to identify potential diagnostic antigens for canine bartonelloses. Continue reading “Research Update CHF 02819 MY2: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses”

Research Update CHF 02819 EY1: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses

During the past 4 years, with support from the American Kennel Club Canine Health Foundation, our research group has documented that current serological assays lack sensitivity, specificity, or both for assessing exposure to Bartonella spp. in dogs. Thus, when used diagnostically for an individual dog or epidemiologically for the detection of Bartonella spp. antibodies in dog populations, results would be inaccurate due to false-negative (poor sensitivity) or false positive (poor specificity) IFA testing. Continue reading “Research Update CHF 02819 EY1: Identification of Bartonella henselae In Vivo Induced Antigens for Development of a Reliable Serodiagnostic Assay for Canine Bartonelloses”

Research Update CHF 02528 Final: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases

Diagnostic tests based on the detection of DNA from harmful organisms in clinical samples have revolutionized veterinary medicine in the last decades. Currently, diagnostic panels for several
vector-borne diseases (VBDs) are available through universities and private labs in the USA and abroad. However, the vast majority of results from sick dogs are negative, which frustrates veterinarians and dog owners trying to reach a definitive diagnosis. Continue reading “Research Update CHF 02528 Final: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases”

Research Update CHF 02553 Final: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens

We developed a comprehensive method for detecting infectious diseases in dogs, taking the guesswork out of determining which tests to use for diagnosis, and potentially improving disease surveillance because of the comprehensive nature of the test. The method is a targeted next-generation sequencing (NGS) assay, which takes advantage of the amount of data that can be generated with NGS but also includes a PCR step prior to sequencing. This reduces costs associated with the sequencing and provides adequate turn-around time for diagnostic use. Continue reading “Research Update CHF 02553 Final: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens”

Research Update CHF 02528 EY3: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases

Diagnostic tests based on the detection of DNA from harmful organisms in clinical samples have revolutionized veterinary medicine in the last decades. Currently, diagnostic panels for several vectorborne diseases (VBDs) are available through universities and private labs in the USA and abroad. Continue reading “Research Update CHF 02528 EY3: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases”

Research Update CHF 02553 EY2: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens

We proposed to develop a comprehensive method for detection of infectious diseases of dogs, taking the guesswork out of determining which tests to use for diagnosis, and potentially improving disease surveillance because of the comprehensive nature of the test. This method takes advantage of the amount of data that can be generated with next-generation sequencing (NGS) but will be performed in a way to keep costs down and maintain adequate turn-around time for diagnostic use. Continue reading “Research Update CHF 02553 EY2: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens”

Research Update CHF 02528 MY2: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases

Despite the wide availability of tick-borne disease panels at laboratories in the USA and abroad, most results from sick dogs are negative, which frustrates veterinarians and dog owners trying to reach a definitive diagnosis. Continue reading “Research Update CHF 02528 MY2: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases”

Research Update CHF 02528 MY3: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases

Despite the wide availability of tick-borne disease panels at laboratories in the USA and abroad, most results from sick dogs are negative, which frustrates veterinarians and dog owners trying to reach a definitive diagnosis. Continue reading “Research Update CHF 02528 MY3: Developing a Next Generation Sequencing Diagnostic Platform for Tick-Borne Diseases”

Research Update Mid-year 3 CHF 02519: Prevalence of Bartonella spp. Infection in Dogs with Cardiac and Splenic Hemangiosarcomas Within and Between Geographic Locations

We are on track to accomplish all of our aims for this study. We were able to obtain the initial set of samples on April 26, 2018 so we had a short delay in starting this study. We have now completed all Year I study aims, with the exception of immunohistochemistry and FISH localization of Bartonella organisms within various cell types. An unanticipated complication arose that the mouse monoclonal antibody was no longer being made commercially. B. henselae specific FISH probes have been designed and validation of FISH probes are in-progress. IHC is also in- progress. All qPCR and ddPCR have been completed at this time and samples are waiting for FISH and IHC analysis.

Continue reading “Research Update Mid-year 3 CHF 02519: Prevalence of Bartonella spp. Infection in Dogs with Cardiac and Splenic Hemangiosarcomas Within and Between Geographic Locations”